How Do I Start Using a Protein Expression Service?
Tuesday , 30, August 2022 Marketing and Advertising Comments Off on How Do I Start Using a Protein Expression Service?If you want to create a protein, but don't have the time or resources to do it yourself, there are plenty of services out there that can help. Protein expression services offer a way to produce high quality recombinant proteins in large quantities at low cost. Here's how to set one up:
1. Decide what you want your product to do. A protein expression service can produce a variety of recombinant proteins, from antibodies and enzymes to growth factors and cytokines. You can also learn more about recombinant protein by visiting at: https://www.bosterbio.com/services/recombinant-protein-expression-service.
Image Source Google
2. Get an estimate of your production costs. This will depend on the type of protein you're producing, the quantity required, and the complexity of the expression protocol.
3. Choose a production platform. There are several types of protein expression services available, including bacterial systems and mammalian cells in culture. Whichever route you choose, make sure you find out which reagents and tools are available for use in your chosen system.
4. Get started! The first step is to identify the genes you'll need to express your proteins. You can either purchase full open source vectors or use gene synthesis kits (which often come with guidance materials).
Once you've got your genes, it's time to design your expression vector(s ). You can either purchase a ready-made vector or design your own. I recommend using the latter option since it's more flexible and fun! (plus, you'll learn a lot!). Once your vectors are designed, they need to be cloned into a plasmid vector. If you're adding multiple genes to make up your expression construct, you'll need to take precautions to avoid them being co-expressed.